Exploring the Dynamic World of Membrane Systems and Biocatalysis

Manuel Etzkorn

Manuel Etzkorn

To understand life on a molecular level, it is of fundamental importance to study structure, interactions, and dynamics of life's key players – proteins, nucleic acids, and lipids – in their native environment. NMR spectroscopy has a unique potential to provide high spatial and temporal resolution of the target molecules in complex environments. However, sample preparation, low sensitivity, and ensemble averaging pose clear limitations in increasingly complex systems.
The presentation will summarize our recent contributions to overcome central restrictions via the development and application of novel methods. This will include our optimized isotope-labelling1, sample-preparation2, and polarization-usage3 strategies as well as selective hyperpolarization via functionalized ligands4.
Furthermore, it will be discussed how the application of the developed methods can provide new insights into: (i) membrane-induced aggregation of the Parkinson’s associated protein α--synuclein5, (ii) signalling of central membrane receptors6,7, and (iii) DNA-mediated biocatalysis8.

[1] Dubey, A. et al. (2021) Angew. Chem. 60, 13783.
[2] Hagn, F. et al. (2013) J. Am. Chem. Soc. 135, 1919
[3] Viegas, A. et al. (2016) J. Biomol. NMR 64, 9.
[4] Viennet, T. et al. (2016) Angew. Chem. 55, 10746.
[5] Viennet, T. et al. (2018) Commun. Biol. 1, 44.
[6] Yi, T. et al. (2014) PNAS 111 ) E2182.
[7] Viegas, A. et al. (2020) Structure 28, 54.
[8] Borggräfe, J. et al. (2022) Nature 601, 144.

 

Go back